Diabetes Care Range
Our central lab products for diabetes include:
- Beta-Hydroxybutyrate LiquiColor Assay - Used to detect ketones to idetify patients at risk of or suffering from diabetic ketoacidosis amongst many other clincial applications.
- STAT-Site M B-HB analyzer - With results within 80 seconds.
- Glycated Serum Protein LiquiColor Assay - Offers a 2-3 week indicator of average blood glucose. This closes the information gap between daily glucose levels and monthly HbA1c testing.
Diabetes is a non-communicable disease characterised by the body’s inability to regulate blood sugar (glucose) levels due to an inability to produce or respond to a hormone called insulin.
Type 1 Diabetes is caused by the pancreas not producing any insulin either due to the body destroying the cells that produce insulin as a result of an auto immune disease or as a result of damage to the pancreas.
Type 2 Diabetes is caused by the body not being able to produce enough insulin or the body cannot respond to the insulin it does produce.
Long term exposure to high glucose levels in the blood is toxic to your tissues and organs. The inability to use glucose or store glucose due to lack of insulin or the body not responding correctly to insulin is therefore significant in terms of your health. This glucose toxicity damages blood vessels and nerves and results in damage or failure of the parts of the body supplied by those nerves and blood vessels. Parts of the body that can be affected are the heart, brain, kidneys, nerves, eyes and skin. For these reasons it is vital that all diabetes patients recieve quality care.
EKF Diagnostics offer a wide range of products for the monitoring and diagnosis of diabetes, such as our point of care analysers and our Stanbio Chemistry reagents.
Beta-Hydroxybutyrate LiquiColor Assay
The Stanbio Chemistry LiquiColor® Beta-Hydroxybutyrate reagent is an enzymatic assay used by many of the most respected healthcare systems. B-Hydroxybutyrate (B-HB) can be run on an open channel of a laboratory analyser. The reagent can quantifiably detect the presence of ketones in patients with suspected diabetic ketoacidosis.
In addition, B-HB can be used to clinically diagnose and monitor the disease status or severity of alcoholism, glycogen storage disease, high fat/low carbohydrate diets, pregnancy, alkalosis, ingestion of isopropyl alcohol, and salicylate poisoning.
WHAT IS KETOSIS?
When the body begins to break down its stored fats in response to a low supply of energy (glucose) it produces the ketone ß-Hydroxybutyrate, which is further catabolised into acetoacetate and then into acetone.
Ketones (like glucose) can be tested or monitored in either urine or blood. Many hospitals still use Acetest or Ketostix for detecting and monitoring ketosis and ketoacidosis. This nitroprusside urine method produces a qualitative assessment of ketosis and ketoacidosis by detecting both acetoacetate and acetone. However nitroprusside methods do not detect B-HB.
B-HB is a better test for ketosis and ketoacidosis than nitroprusside methods for a number of reasons:
- The nitroprusside method detects less than 22% of the ketones present in ketosis. B-HB is the main ketone produced by the body (78%).
- B-HB demonstrates excellent stability, making it the most reliable indicator of clinically relevant ketosis and ketoacidosis.
- During ketosis, B-HB levels increase more than levels of acetone and acetoacetate, clearly indicating the patient's trend in metabolic status.
- Quantitative, objective Beta-Hydroxybutyrate results provide a better tool for differentiating metabolic acidosis and monitoring therapy.
People with type 1 diabetes may be at risk when they do not have enough insulin, a hormone the body uses to break down sugar (glucose) in the blood for energy. When the body senses glucose is not available, fat is broken down instead.
As fats are broken down, acids called ketones build up in the blood and urine. Ketones are poisonous in high levels. This condition is called ketoacidosis.
Blood glucose levels rise (usually higher than 300 mg/dL) because the liver makes glucose to try to combat the problem. However the cells cannot pull in that glucose without insulin.
Symptoms can include rapid deep breathing, dry skin and mouth, flushed face, fruity smelling breath, nausea, vomiting and stomach pain. Other symptoms that can occur are; Abdominal pain, breathing difficulty while lying down, decreased appetite, decreased conciousness, muscle stifness and aches, dulled senses that may worsen to a coma and frequent urination and thirst that lasts throughout the day.
FIND OUT MORE
To find out more about how B-HB can improve diagnosis in your laboratory using the Stanbio Chemistry LiquiColor Beta-Hydroxybutyrate chemistry reagent contact us today.
Click on the tabs on the right of this page to view a video from Dr James Nichols, (formerly of Tufts University, Boston) Now Professor of Pathology, Microbiology & Immunology and Director of Clinical Chemistry at Vanderbilt University School of Medicine, Nashville, TN.
STAT-Site M B-HB
The STAT-Site® M ß-HB system is intended for the quantitative determination of ß-Hydroxybutyrate in serum or plasma, aiding in the diagnosis and monitoring of ketoacidosis. It is composed of a reflectance photometer and dry-reagent test strips.
- Quantitative ß-HB results provide the best and earliest indicator of ketosis and resolution of ketoacidosis (DKA).
- Reliable: ß-Hydroxybutyrate is 78% of all available ketones and provide a better tool for differentiating metabolic acidosis and monitoring response to therapy.
- Improved Clinical Outcomes: resulting in higher quality of patient care while reducing ER/ICU patient stay.
- Fast: new platform delivers results within 80 seconds.
- No lag time due to testing Serum or Plasma samples.
Methodology: Reflectance photometry
Principle: ß-Hydroxybutyrate dehydrogenase
Sample Size: 10µL
Range: 0.01 - 2.0 mmol/L (without dilution)
Calibration: Automatic (CODE Key)
Lot specific CODE Key included in every box of test strips.
To find out more about the STAT-Site Beta-Hydroxybutyrate photometer make an enquiry now.
Glycated Serum Protein LiquiColor® Assay
The Glycated Serum Protein (GSP) LiquiColor® test serves as a 2-3 week indicator of average blood glucose closing the information gap between daily blood glucose testing and the 2-3 month HbA1c reading. GSP serves as an accurate intermediate marker of glycemia in instances where HbA1c may be of limited value such as pregnancy, reduced RBC lifespan and hemodialysis.
The difference between actual measured HbA1c and predicted HbA1c from glycated serum protein is called the glycation gap. Measuring HbA1c and GSP together offers improved diagnostic accuracy by more reliably predicting complications of diabetes including nephropathy and retinopathy.
Provides superior specificity and accuracy compared to fructosamine assays (NBT method) for monitoring and assessment of short-term to medium-term (past 2-3 week period) average blood glucose levels. Complementary to HbA1c in diagnosis and screening of diabetes.
Studies suggest that combining GSP results with HbA1c measurements provides a better assessment of long term risk of diabetic complications.
Glycated serum protein vs conventional fructosamine assay
The Stanbio Chemistry GSP test utilizes the specificity of fructosyl-amino oxidase to eliminate significant interferences.
Enzymatic specificity and accuracy
The Stanbio Chemistry GSP test enzymatic method is more reliable and specific than the older non-enzymatic fructosamine NBT method. This method eliminates the inaccuracies caused by non-glycated protein reducing substances which interfere with the NBT fructosamine method.
Liquid stable kit and calibrator with a wide variety of instrument parameters for most open clinical chemistry systems. For use on a variety of clinical chemistry analyzers.